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Product Name
VPS34 antibody
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Description
VPS34 Rabbit Polyclonal antibody. Positive WB detected in PC-3 cells, HeLa cells, human brain tissue, human testis tissue, mouse brain tissue, mouse lung tissue, mouse testis tissue, rat brain tissue. Positive IHC detected in human heart tissue. Positive IF detected in PC-3 cells. Observed molecular weight by Western-blot: 100 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
hVps34 antibody; PI 3 Kinase Class 3 antibody; PI 3-kinase p100 antibody; PI3K p100 antibody; PI3K type 3 antibody; PIK3C3 antibody; PtdIns 3 kinase type 3 antibody; VPS34 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of VPS34 recombinant protein (Accession Number: NM_002647). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:116782(PIK3C3 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human heart using Catalog No:116782(PIK3C3 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:116782(PIK3C3 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of PC-3 cells using Catalog No:116782(PIK3C3 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
PIK3C3(Phosphatidylinositol 3-kinase catalytic subunit type 3) is also named as VPS34 and belongs to the PI3/PI4-kinase family.It is a catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes.It can form a dimer(PMID:20339072).
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References
- Xu D, Zhang T, Xiao J. Modification of BECN1 by ISG15 plays a crucial role in autophagy regulation by type I IFN/interferon. Autophagy. 11(4):617-28. 2015.
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