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Product Name
VPS33A antibody
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Description
VPS33A Rabbit Polyclonal antibody. Positive IP detected in HepG2 cells. Positive WB detected in human brain tissue, HepG2 cells, human cerebellum tissue, NIH/3T3 cells. Observed molecular weight by Western-blot: 68 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ22395 antibody; FLJ23187 antibody; hVPS33A antibody; VPS33A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of VPS33A recombinant protein (Accession Number: NM_022916). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:116780(VPS33A antibody) at dilution of 1:500
IP Result of anti-VPS33A (IP:Catalog No:116780, 4ug; Detection:Catalog No:116780 1:600) with HepG2 cells lysate 1600ug.
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Background
VPS33A is a Sec1/Munc18 family member and is a component of the homotypic fusion and protein sorting (HOPS)-tethering complex. The mammalian HOPS complex comprises six subunits (VPS11, VPS16, VPS18, VPS33A, VPS39, and VPS41) (PMID: 23901104). This complex is required for late endosome-lysosome and autophagosome-lysosome fusion (PMID: 24554770). VPS33A plays a role in vesicular transport to the lysosomal compartment.
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References
- Khatter D, Raina VB, Dwivedi D, Sindhwani A, Bahl S, Sharma M. The small GTPase Arl8b regulates assembly of the mammalian HOPS complex on lysosomes. Journal of cell science. 128(9):1746-61. 2015.
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