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Product Name
VPS28 antibody
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Description
VPS28 Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in mouse brain tissue. Observed molecular weight by Western-blot: 28-30 kDa
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Tested applications
ELISA, IP, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ESCRT I complex subunit VPS28 antibody; H Vps28 antibody; VPS28 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of VPS28 recombinant protein (Accession Number: NM_016208). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:116779(VPS28 Antibody) at dilution of 1:600
IP Result of anti-VPS28 (IP:Catalog No:116779, 4ug; Detection:Catalog No:116779 1:500) with mouse brain tissue lysate 4400ug.
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Background
VPS28 is a subunit of the ESCRT-I complex (endosomal sorting complex I required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. The ESCRT-I complex is composed of VPS23 (also known as TSG101), VPS28, VPS37, and MVB12A or MVB12B. VPS28 interacts with VPS23. Two alternative transcripts encoding different VPS28 isoforms have been described.
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