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Product Name
VPS11 antibody
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Description
VPS11 Rabbit Polyclonal antibody. Positive FC detected in HepG2 cells. Positive IF detected in HepG2 cells. Positive WB detected in human brain tissue, HEK-293 cells, human heart tissue, human kidney tissue, mouse pancreas tissue. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 108-112 kDa
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Tested applications
ELISA, WB, IF, FC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
END1 antibody; hVPS11 antibody; PEP5 antibody; RING finger protein 108 antibody; RNF108 antibody; VPS11 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of VPS11 recombinant protein (Accession Number: NM_021729). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:116770(VPS11 antibody) at dilution of 1:500
Immunofluorescent analysis of HepG2 cells, using VPS11 antibody Catalog No:116770 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-VPS11 (IP:Catalog No:116770, 4ug; Detection:Catalog No:116770 1:500) with HEK-293 cells lysate 1480ug.
1X10^6 HepG2 cells were stained with 0.2ug VPS11 antibody (Catalog No:116770, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. In yeast, Vps proteins are involved in the trafficking of endocytic and biosynthetic proteins to the vacuole, which functionally resembles the lysosome of higher organisms. VPS11 is the human homolog of the yeast class C Vps11 protein, a subunit of HOPS (homotypic fusion and protein transport) complex. Mammalian Vps11 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.
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References
- van der Kant R, Fish A, Janssen L. Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L. Journal of cell science. 126(Pt 15):3462-74. 2013.
- van der Kant R, Jonker CT, Wijdeven RH. Characterization of the Mammalian CORVET and HOPS Complexes and Their Modular Restructuring for Endosome Specificity. The Journal of biological chemistry. 290(51):30280-90. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"