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Product Name
Visfatin antibody
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Description
Visfatin Rabbit Polyclonal antibody. Positive IHC detected in human ovary tumor tissue. Positive IP detected in mouse heart tissue. Positive WB detected in mouse heart tissue, HL-60 cells, mouse liver tissue, rat liver tissue, zebrafish tissue. Observed molecular weight by Western-blot: 46-52 kDa
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Tested applications
ELISA, IHC, WB, IP
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Species reactivity
Human,Mouse,Rat, Zebrafish; other species not tested.
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Alternative names
1110035O14Rik antibody; DKFZp666B131 antibody; NAmPRTase antibody; NAMPT antibody; PBEF antibody; PBEF1 antibody; Pre B cell enhancing factor antibody; VISFATIN antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Visfatin recombinant protein (Accession Number: BC020691). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:116758(NAMPT antibody) at dilution of 1:50 (under 10x lens)
mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:116758(NAMPT antibody) at dilution of 1:1000
IP Result of anti-NAMPT (IP:Catalog No:116758, 3ug; Detection:Catalog No:116758 1:1000) with mouse heart tissue lysate 4000ug.
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Background
Nicotinamide phosphoribosyltransferase(NAMPT) has two usual synonyms termed Visfatin and PBEF. Its primary role is to catalyze the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD, which is the rate limiting component in the mammalian NAD biosynthesis pathway. NAMPT is localized in cytoplasm and expressed in large amounts in bone marrow, liver tissue, and muscle tissues. NAMPT inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis. NAMPT levels are altered in plasma of patients with type 2 diabetes mellitus (T2DM), and it is now evidenced that NAMPT may plays a role in lipid metabolism.
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References
- Tummala KS, Gomes AL, Yilmaz M. Inhibition of de novo NAD(+) synthesis by oncogenic URI causes liver tumorigenesis through DNA damage. Cancer cell. 26(6):826-39. 2014.
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