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Product Name
VIPAS39 antibody
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Description
VIPAS39 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, A431 cells, HEK-293 cells, HT-1080 cells, human kidney tissue, L02 cells. Positive IF detected in Hela cells, HEK-293 cells, HepG2 cells. Observed molecular weight by Western-blot: 51 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
C14orf133 antibody; FLJ12707 antibody; hSPE 39 antibody; Protein spe 39 homolog antibody; VIPAR antibody; VIPAS39 antibody; VPS33B interacting protein antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of VIPAS39 recombinant protein (Accession Number: NM_001193317). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IF: 1:10-1:100
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:116756(C14orf133 antibody) at dilution of 1:300
Immunofluorescent analysis of Hela cells, using C14orf133 antibody Catalog No:116756 at 1:25 dilution and FITC- labeled FITC-Donkey anti-Rabbit IgG (green).
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Background
VIPAS39 (also known as C14orf133 or SPE-39) is a binding protein to Vps33B, one of the subunit in the mammalian HOPS complex. VIPAS39 may be involved in endosomal maturation or fusion (PMID: 23918659). Mutations in Vps33B and VIPAS39 cause arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome, which is a rare autosomal recessive multisystem disorder associated with abnormalities in polarized liver and kidney cells (PMID: 20190753).
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