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Product Name
Vinculin antibody
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Description
Vinculin Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HepG2 cells, NIH/3T3 cells. Observed molecular weight by Western-blot: 117 kDa
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Tested applications
ELISA, WB
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
CMD1W antibody; Metavinculin antibody; MVCL antibody; VCL antibody; Vinculin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Vinculin recombinant protein (Accession Number: NM_003373). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116755(Vinculin Antibody) at dilution of 1:2000
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Background
Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It plays an important role in cell morphology and locomotion.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"