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Vinculin antibody

Cat.#: 107541

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Special Price 239.0 USD

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Product Information

  • Product Name
    Vinculin antibody
  • Documents
  • Description
    Vinculin Mouse Monoclonal antibody. Positive WB detected in HeLa cells, pig heart tissue, rat heart tissue. Positive IHC detected in human breast cancer tissue, human prostate cancer tissue. Positive IF detected in HeLa cells. Positive FC detected in HeLa cells. Observed molecular weight by Western-blot: 117 kDa
  • Tested applications
    ELISA, WB, IF, IHC, FC
  • Species reactivity
    Human, Mouse, Rat, Pig; other species not tested.
  • Alternative names
    CMD1W antibody; Metavinculin antibody; MVCL antibody; VCL antibody; Vinculin antibody
  • Isotype
    Mouse IgG1
  • Preparation
    This antibody was obtained by immunization of Vinculin recombinant protein (Accession Number: NM_003373). Purification method: Protein G purified.
  • Clonality
    Monoclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:2000-1:20000

    IHC: 1:50-1:500

    IF: 1:50-1:500

  • Validations

    Various cells were subjected to SDS PAGE followed by western blot with Catalog No:117346(Vinculin Antibody) at dilution of 1:5000

    Various cells were subjected to SDS PAGE followed by western blot with Catalog No:117346(Vinculin Antibody) at dilution of 1:5000

    Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:117346(Vinculin Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:117346(Vinculin Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:117346(Vinculin Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:117346(Vinculin Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:117346(Vinculin Antibody) at dilution of 1:200 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:117346(Vinculin Antibody) at dilution of 1:200 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)

    1X10^6 HeLa cells were stained with 0.2ug Vinculin antibody (Catalog No:117346, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500.

    1X10^6 HeLa cells were stained with 0.2ug Vinculin antibody (Catalog No:117346, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500.

  • Background
    Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It plays an important role in cell morphology and locomotion.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"