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Product Name
Vimentin antibody
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Description
Vimentin Mouse Monoclonal antibody. Positive IHC detected in human renal cell carcinoma tissue. Positive IF detected in HeLa cells. Positive WB detected in Jurkat cells. Observed molecular weight by Western-blot: 55-60 kDa
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
FLJ36605 antibody; VIM antibody; Vimentin antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of Vimentin recombinant protein (Accession Number: XM_006717500). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:107540(Vimentin Antibody) at dilution of 1:2000
Immunohistochemistry of paraffin-embedded human renal cell carcinoma tissue slide using Catalog No:107540(Vimentin Antibody) at dilution of 1:500 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunofluorescent analysis of HeLa cells using Catalog No:107540(Vimentin Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
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Background
Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.
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References
- Muir KR, Lima MJ, Docherty HM. Krüppel-Like Factor 4 Overexpression Initiates a Mesenchymal-to-Epithelial Transition and Redifferentiation of Human Pancreatic Cells following Expansion in Long Term Adherent Culture. PloS one. 10(10):e0140352. 2015.
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