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Product Name
Villin antibody
- Documents
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Description
Villin Rabbit Polyclonal antibody. Positive WB detected in mouse kidney tissue, mouse colon tissue, mouse liver tissue. Positive IP detected in mouse kidney tissue. Positive IF detected in COLO 320 cells. Positive IHC detected in human small intestine tissue, human colon cancer tissue. Observed molecular weight by Western-blot: 93 kDa
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Tested applications
ELISA, IF, IP, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
VIL antibody; VIL1 antibody; Villin antibody; villin 1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Villin recombinant protein (Accession Number: BC017303). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:116752(VIL1 antibody) at dilution of 1:2000
IP Result of anti-VIL1 (IP:Catalog No:116752, 4ug; Detection:Catalog No:116752 1:300) with mouse kidney tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human small intestine tissue slide using Catalog No:116752(VIL1 Antibody) at dilution of 1:2000 (under 10x lens).
Immunohistochemistry of paraffin-embedded human small intestine tissue slide using Catalog No:116752(VIL1 Antibody) at dilution of 1:2000 (under 40x lens).
Immunofluorescent analysis of COLO 320 cells using Catalog No:116752(VIL1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Villin 1 (VIL1) is a 95-kd F-actin bundling and severing protein and its expression is restricted to epithelial cells with a brush border, like epithelial cells of the intestinal mucosa, gall bladder, renal proximal tubules and ductuli efferentes of the testis. VIL1 has been reported to be an epithelial cell-specific anti-apoptotic protein, and to have an important function in regulating actin dynamics, cell morphology, epithelial-to-mesenchymal transitions, cell migration and cell survival. In addition, VIL1 is a useful diagnostic marker for of various cancer, like cervical and endometrial adenocarcinomas, renal cell carcinoma. VIL1 was recently identified as a novel biomarker predictive for postoperative recurrence and poorer prognosis of high serum AFP associated HCC.
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