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Product Name
VILIP-1 antibody
- Documents
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Description
VILIP-1 Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, HeLa cells, human brain tissue, mouse tongue tissue, rat brain tissue. Positive IP detected in mouse brain tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 22-24 kDa
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Tested applications
ELISA, WB, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
HippOCalcin like protein 3 antibody; HLP3 antibody; HPCAL3 antibody; HUVISL1 antibody; VILIP antibody; VILIP 1 antibody; VILIP1 antibody; visinin like 1 antibody; Visinin like protein 1 antibody; VISL1 antibody; VLP 1 antibody; VSNL1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of VILIP-1 recombinant protein (Accession Number: NM_001366803). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IF: 1:10-1:100
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:116751(VSNL1 antibody) at dilution of 1:1500
IP Result of anti-VSNL1 (IP:Catalog No:116751, 3ug; Detection:Catalog No:116751 1:1000) with mouse brain tissue lysate 2640ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:116751(VSNL1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
VSNL1 represents visinin-like 1
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References
- Liebl MP, Kaya AM, Tenzer S. Dimerization of visinin-like protein 1 is regulated by oxidative stress and calcium and is a pathological hallmark of amyotrophic lateral sclerosis. Free radical biology & medicine. 72:41-54. 2014.
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