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VGAT antibody

Cat.#: 116743

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Product Information

  • Product Name
    VGAT antibody
  • Documents
  • Description
    VGAT Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in mouse brain tissue. Observed molecular weight by Western-blot: 57 kDa
  • Tested applications
    ELISA, WB, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    GABA and glycine transporter antibody; hVIAAT antibody; SLC32A1 antibody; Vesicular GABA transporter antibody; VGAT antibody; VIAAT antibody
  • Immunogen
    Human SLC32A1 Recombinant protein (GST tag)
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of VGAT recombinant protein (Accession Number: NM_080552). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

  • Validations

    mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:116743(SLC32A1 antibody) at dilution of 1:1000

    mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:116743(SLC32A1 antibody) at dilution of 1:1000

    IP Result of anti-SLC32A1 (IP:Catalog No:116743, 4ug; Detection:Catalog No:116743 1:500) with mouse brain tissue lysate 4000ug.

    IP Result of anti-SLC32A1 (IP:Catalog No:116743, 4ug; Detection:Catalog No:116743 1:500) with mouse brain tissue lysate 4000ug.

  • Background
    SLC32A1, also known as VGAT (vesicular GABA transporter), functions in the uptake of GABA and glycine into synaptic vesicles. GABA (gamma-aminobutyric acid), is the major inhibitory neurotransmitter in the CNS. VGAT transports GABA and glycine into acidic vesicles and localizes to the synaptic vesicle in glycinergic and GABAergic neurons. And VGAT antibodies are useful markers for presynaptic GABAergic and glycinergic neurons.
  • References
    • Vaccaro A, Tauffenberger A, Aggad D, Rouleau G, Drapeau P, Parker JA. Mutant TDP-43 and FUS cause age-dependent paralysis and neurodegeneration in C. elegans. PloS one. 7(2):e31321. 2012.
    • Wang P, Eshaq RS, Meshul CK, Moore C, Hood RL, Leidenheimer NJ. Neuronal gamma-aminobutyric acid (GABA) type A receptors undergo cognate ligand chaperoning in the endoplasmic reticulum by endogenous GABA. Frontiers in cellular neuroscience. 9:188. 2015.
    • Schweikhard ES, Burckhardt BC, Joos F. Role of N-glycosylation in renal betaine transport. The Biochemical journal. 470(2):169-79. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"