-
Product Name
VEPH1 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to VEPH1
-
Tested applications
WB, IHC
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
VEPH1 antibody; MELT antibody; VEPH antibody; ventricular zone expressed PH domain containing 1 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 448-547 of human VEPH1 (NP_078897.2).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - VEPH1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using VEPH1 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 5s.
-
Background
Interacts with TGF-beta receptor type-1 (TGFBR1) and inhibits dissociation of activated SMAD2 from TGFBR1, impeding its nuclear accumulation and resulting in impaired TGF-beta signaling. May also affect FOXO, Hippo and Wnt signaling.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"