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Product Name
VEGFR-1/FLT-1 antibody
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Description
VEGFR-1/FLT-1 Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, human placenta tissue, mouse lung tissue. Positive IP detected in A549 cells. Positive IF detected in HeLa cells. Positive FC detected in HUVEC cells. Observed molecular weight by Western-blot: 55-60 kDa
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Tested applications
ELISA, WB, IP, IF, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLT antibody; FLT 1 antibody; FLT1 antibody; FLT-1 antibody; Fms like tyrosine kinase 1 antibody; FRT antibody; sflt1 antibody; Tyrosine protein kinase FRT antibody; VEGF receptor 1 antibody; VEGFR 1 antibody; VEGFR1 antibody; VEGFR-1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of VEGFR-1/FLT-1 recombinant protein (Accession Number: NM_001159920). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:116739(VEGFR1, FLT1 antibody) at dilution of 1:800
IP Result of anti-VEGFR1, FLT1 (IP:Catalog No:116739, 5ug; Detection:Catalog No:116739 1:500) with A549 cells lysate 3300ug.
Immunofluorescent analysis of HeLa cells using Catalog No:116739(VEGFR1, FLT1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 HUVEC cells were stained with 0.2ug VEGFR1, FLT1 antibody (Catalog No:116739, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
Vascular endothelial growth factor receptor-1 (VEGFR-1, FLT-1) is a receptor tyrosine kinase belonging to the VEGFR family. VEGF is a key regulator of physiological angiogenesis and has also been implicated in pathological angiogenesis associated with tumors, intraocular neovascular disorders and other conditions. The biological effects of VEGF are mediated by VEGFR-1 and VEGFR-2. Both the two receptors have seven immunoglobulin-like repeats in the extracellular domain, a single transmembrane region and a tyrosine kinase domain. VEGFR-1 binds VEGFA, PIGF and VEGFB, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. Two isoforms of VEGFR-1 exist, a full-length transmembrane form and a short souble form (sVEGFR-1) consisting of only the extracellular ligand-binding domain. (PMID: 12778165; 17109193; 8806634)
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References
- Xi S, Shi M, Jiang X. The effects of Tao-Hong-Si-Wu on hepatic necroinflammatory activity and fibrosis in a murine model of chronic liver disease. Journal of ethnopharmacology. 180:28-36. 2016.
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