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Product Name
VEGFB Polyclonal Antibody
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Description
Polyclonal antibody to VEGFB
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse
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Alternative names
VEGFB antibody; VEGFL antibody; VRF antibody; vascular endothelial growth factor B antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-207 of human VEGFB (NP_003368.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:1000 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - VEGFB Polyclonal Antibody
Western blot analysis of extracts of U-87MG cells, using VEGFB antibody at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 90s.
Immunohistochemistry - VEGFB Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human mammary cancer using VEGFB antibody at dilution of 1:200 (40x lens).
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Background
Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"