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Product Name
VDAC3 Polyclonal Antibody
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Description
Polyclonal antibody to VDAC3
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Tested applications
WB, IF
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Species reactivity
Human
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Alternative names
VDAC3 antibody; HD-VDAC3 antibody; VDAC-3 antibody; voltage dependent anion channel 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-283 of human VDAC3 (NP_005653.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:1000 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - VDAC3 Polyclonal Antibody
Western blot analysis of extracts of A-431 cells, using VDAC3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
Immunofluorescence - VDAC3 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using VDAC3 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Background
Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"