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Product Name
VDAC1/Porin antibody
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Description
VDAC1/Porin Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, DU 145 cells, HeLa cells, HL-60 cells, human heart tissue, Jurkat cells, mouse brain tissue, mouse heart tissue, mouse kidney tissue. Positive IP detected in mouse skeletal muscle tissue. Positive IHC detected in human pancreas cancer tissue, human colon tissue, human pancreas tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 31kd
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
hVDAC1 antibody; Plasmalemmal porin antibody; Porin antibody; PORIN 31 HL antibody; Porin 31HL antibody; Porin 31HM antibody; VDAC antibody; VDAC 1 antibody; VDAC1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of VDAC1/Porin recombinant protein (Accession Number: NM_001401017). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:117345(VDAC1 antibody) at dilution of 1:500
IP Result of anti-VDAC1 (IP:Catalog No:117345, 3ug; Detection:Catalog No:117345 1:1000) with mouse skeletal muscle tissue lysate 7500ug.
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:117345(VDAC1 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:117345(Porin Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
VDAC1, also named as VDAC, Porin 31HM, Porin 31HL and Plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. It is a 31kd membrane protein conserved in chimpanzee, dog, cow, mouse, rat, chicken, and zebrafish.
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References
- Chen H, Gao W, Yang Y. Inhibition of VDAC1 prevents Ca²⁺-mediated oxidative stress and apoptosis induced by 5-aminolevulinic acid mediated sonodynamic therapy in THP-1 macrophages. Apoptosis : an international journal on programmed cell death. 19(12):1712-26. 2014.
- Liu C, Lin JJ, Yang ZY, Tsai CC, Hsu JL, Wu YJ. Proteomic study reveals a co-occurrence of gallic acid-induced apoptosis and glycolysis in B16F10 melanoma cells. Journal of agricultural and food chemistry. 62(48):11672-80. 2014.
- Gao S, Li H, Feng XJ. α-Enolase plays a catalytically independent role in doxorubicin-induced cardiomyocyte apoptosis and mitochondrial dysfunction. Journal of molecular and cellular cardiology. 79:92-103. 2015.
- Saunders C, Smith L, Wibrand F. CLPB variants associated with autosomal-recessive mitochondrial disorder with cataract, neutropenia, epilepsy, and methylglutaconic aciduria. American journal of human genetics. 96(2):258-65. 2015.
- Lyu J, Zheng G, Chen Z. Sepsis-induced brain mitochondrial dysfunction is associated with altered mitochondrial Src and PTP1B levels. Brain research. 1620:130-8. 2015.
- Rafiq J, Duno M, Østergaard E. Exercise Intolerance and Myoglobinuria Associated with a Novel Maternally Inherited MT-ND1 Mutation. JIMD reports. 2015.
- Liao H, Bao X, Zhu J. O-Alkylated derivatives of quercetin induce apoptosis of MCF-7 cells via a caspase-independent mitochondrial pathway. Chemico-biological interactions. 242:91-8. 2015.
- Xu C, Yi C, Wang H, Bruce IC, Xia Q. Mitochondrial nitric oxide synthase participates in septic shock myocardial depression by nitric oxide overproduction and mitochondrial permeability transition pore opening. Shock (Augusta, Ga.). 37(1):110-5. 2012.
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