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Product Name
VDAC1/Porin antibody
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Description
VDAC1/Porin Rabbit Polyclonal antibody. Positive IHC detected in human heart tissue, human liver tissue. Positive FC detected in HepG2 cells. Positive WB detected in HepG2 cells, human skeletal muscle tissue, mouse liver tissue. Positive IP detected in A431 cells. Observed molecular weight by Western-blot: 31-33 kDa
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Tested applications
ELISA, WB, IHC, FC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
hVDAC1 antibody; Plasmalemmal porin antibody; Porin antibody; PORIN 31 HL antibody; Porin 31HL antibody; Porin 31HM antibody; VDAC antibody; VDAC 1 antibody; VDAC1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_003374). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:117344(VDAC1 antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human heart using Catalog No:117344(VDAC1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:117344(VDAC1 antibody) at dilution of 1:50 (under 40x lens)
1X10^6 HepG2 cells were stained with 0.2ug VDAC1 antibody (Catalog No:117344, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
IP Result of anti-VDAC1 (IP:Catalog No:117344, 4ug; Detection:Catalog No:117344 1:500) with A431 cells lysate 2400ug.
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Background
VDAC1, also named as VDAC, Porin 31HM, Porin 31HL and Plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. It is a 31kd membrane protein conserved in chimpanzee, dog, cow, mouse, rat, chicken, and zebrafish. This antibody is specific to VDAC1.
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