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Product Name
VASP antibody
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Description
VASP Rabbit Polyclonal antibody. Positive IHC detected in human testis tissue, human heart tissue, human kidney tissue, human lung tissue, human skin cancer tissue, human spleen tissue. Positive IF detected in HepG2 cells. Positive FC detected in HepG2 cells. Positive WB detected in A431 cells, A549 cells, HEK-293 cells, HeLa cells, mouse spleen tissue. Positive IP detected in mouse spleen tissue. Observed molecular weight by Western-blot: 46 kDa, 50 kDa
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Tested applications
ELISA, WB, IHC, IF, FC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
VASP antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of VASP recombinant protein (Accession Number: NM_003370). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:116719(VASP antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human testis using Catalog No:116719(VASP antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:116719(VASP antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using VASP antibody Catalog No:116719 at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green).
IP Result of anti-VASP (IP:Catalog No:116719, 4ug; Detection:Catalog No:116719 1:500) with mouse spleen tissue lysate 4800ug.
1X10^6 HepG2 cells were stained with 0.2ug VASP antibody (Catalog No:116719, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
VASP belongs to the Ena/VASP family. Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. VASP plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation. Human platelet activation is inhibited by agents such as prostaglandins and NO donors, which elevate cAMP or cGMP levels. VASP is phosphorylated in human platelets in response to both cAMP- and cGMP-elevating agents, and its phosphorylation correlates with platelet inhibition. VASP is located about 92 kb distal to ERCC1 (126380) and about 300 kb proximal to the myotonic dystrophy protein kinase gene. The antibody is specific to VASP.
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