Rat VEGFR1/FLT-1 (His Tag) recombinant protein

Rat VEGFR1/FLT-1 (His Tag) recombinant protein

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• Material Safety Data Sheets (MSDS)

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, and proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts. Has very high affinity for VEGFA and relatively low protein kinase activity; may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR. Modulates KDR signaling by forming heterodimers with KDR. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leading to the activation of phosphatidylinositol kinase and the downstream signaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Phosphorylates SRC, YES1 and PLCG, and may also phosphorylate CBL. Promotes phosphorylation of AKT1 and PTK2/FAK1 (By similarity).

Vascular endothelial growth factor receptor 1

FLT1; FLT; FLT-1; VEGFR1; VEGFR-1; SFLT1; AI323757

P53767

Flt1; Flt-1; Vegfr1

Human Cells

A DNA sequence encoding the rat FLT1 (P53767) (Met1-Glu758) was expressed with a polyhistidine tag at the C-terminus.

Rat

The recombinant rat FLT1 comprises 747 amino acids and predicts a molecular mass of 84 kDa. The apparent molecular mass of the recombinant protein is approximately 108-114 kDa in SDS-PAGE under reducing conditions due to glycosylation.

> 90 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized ratFLT1-His (Cat: 500481) at 10 μg/ml (100 μl/well) can bind biotinylated mouse VEGFD-Fc (Cat: 501152) with a linear range of 0.125-2.0 μg/ml.