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Product Name
Mouse VCAM-1/CD106 (His Tag) recombinant protein
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Description
Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
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Protein name
Vascular cell adhesion protein 1
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Protein short names
DKFZP779G2333; MGC99561; CD106; VCAM-1; INCAM-100; VCAM1
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Uniprot ID
Q3UPN1
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Gene Name
Vcam1; Vcam-1
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the extracellular domain (Met 1-Glu 698) of mouse VCAM1 (NP_035823.3) was fused with the a polyhistidine tag at the C-terminus.
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Protein Species
Mouse
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Molecular weight
The secreted recombinant mouse VCAM1 consists of 685 amino acids and has a predicted molecular mass of 75.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmVCAM1 is approximately 90-100 kDa due to glycosylation.
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Purity
> 97 % as determined by SDS-PAGE
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Activity
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 10E4 cells/well are added to mouse VCAM1 coated plates (10 μg/ml with 100 μl/well), approximately 70%-80% cells will adhere after 1 hour incubation at RT.
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Validations
Mouse VCAM1 / L1CAM / CD106 Protein (His Tag) SDS-PAGE
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