Human VCAM-1/CD106 (His Tag) recombinant protein

Human VCAM-1/CD106 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Vascular cell adhesion molecule 1 (VCAM-1), also known as CD106, is a cell surface sialoglycoprotein belonging to the immunoglobulin superfamily. Two forms of VCAM-1 with either six or seven extracellular Ig-like domains are generated by alternative splicing, with the longer form predominant. VCAM-1 is an endothelial ligand for very late antigen-4 (VLA-4) and α4ß7 integrin expressed on leukocytes, and thus mediates leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 expression is induced on endothelial cells during inflammatory bowel disease, atherosclerosis, allograft rejection, infection, and asthmatic responses. During these responses, VCAM-1 forms a scaffold for leukocyte migration. VCAM-1 also activates signals within endothelial cells resulting in the opening of an "endothelial cell gate" through which leukocytes migrate. VCAM-1 has been identified as a potential anti-inflammatory therapeutic target, the hypothesis being that reduced expression of VCAM-1 will slow the development of atherosclerosis. In addition, VCAM-1-activated signals in endothelial cells are regulated by cytokines indicating that it is important to consider both endothelial cell adhesion molecule expression and function during inflammatory processes.

Vascular cell adhesion protein 1

DKFZP779G2333; MGC99561; VCAM-1; INCAM-100; CD106; VCAM1

P19320

VCAM1; L1CAM

Human Cells

A DNA sequence encoding the extracellular domain (Phe 25-Pro 697) of human VCAM1 (NP_001069.1) was fused with a polyhistidine tag at the N-terminus.

Human

The recombinant human VCAM1 consists of 689 amino acids and predicts a molecular mass of 76 kDa. As a result of glycosylation, rhVCAM1 migrates as an approximately 110 kDa band in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When cells are added to VCAM1 coated plates (5 μg/mL, 100 μL/well) approximately 60% will adhere for 1 hour incubation at 37℃.