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Product Name
VAPA antibody
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Description
VAPA Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive IF detected in HepG2 cells, HEK-293 cells. Positive IP detected in HEK-293 cells. Positive WB detected in human kidney tissue, HEK-293 cells, HepG2 cells, human brain tissue, mouse skeletal muscle tissue. Observed molecular weight by Western-blot: 33 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
33 kDa VAMP assOCiated protein antibody; hVAP 33 antibody; VAMP A antibody; VAMP assOCiated protein A antibody; VAP 33 antibody; VAP A antibody; VAP33 antibody; VAPA antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of VAPA recombinant protein (Accession Number: NM_194434). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:116714(VAPA antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:116714(VAPA antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:116714(VAPA antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-VAPA (IP:Catalog No:116714, 3ug; Detection:Catalog No:116714 1:1000) with HEK-293 cells lysate 3200ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:116714(VAPA Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
VAPA (Vesicle-associated membrane protein-associated protein A), also known as VAP33, is a 33-kDa, ubiquitously expressed integral membrane protein of the VAMP-associated protein (VAP) family. It is present in the plasma membrane and in intracellular vesicles, playing a role in vesicle trafficking.
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References
- Chong WM, Hsu SC, Kao WT. Phosphoproteomics Identified an NS5A Phosphorylation Site Involved in Hepatitis C Virus Replication. The Journal of biological chemistry. 291(8):3918-31. 2016.
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