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Product Name
V5-tag antibody
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Description
V5-tag Mouse Monoclonal antibody. Positive IP detected in Transfected HEK-293 cells. Positive WB detected in recombinant protein.
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Tested applications
ELISA, WB, IP
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Species reactivity
Human; other species not tested.
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Alternative names
V5 tag antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of Peptide. Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:5000-1:50000
IP: 1:2000-1:20000
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Validations
Western blot of V5-tagged fusion protein with V5 tag monoclonal antibody (Catalog No:117343) at various dilutions.
IP Result of anti-V5-tag (IP:Catalog No:117343, 4ug; Detection:Catalog No:117343 1:5000) with Transfected HEK-293 cells lysate 400ug.
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Background
The antibody is developed against a synthetic 14 aa peptide (GKPIPNPLLGLDST), the V5 tag, which presents in many vectors.
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References
- Li Y, Dong W, Shi Y. Rabies virus phosphoprotein interacts with ribosomal protein L9 and affects rabies virus replication. Virology. 488:216-24. 2016.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"