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Product Name
USP5 Polyclonal Antibody
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Description
Polyclonal antibody to USP5
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
USP5 antibody; ISOT antibody; ubiquitin specific peptidase 5 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human USP5 (NP_003472.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:1000 - 1:2000
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Validations
Western blot - USP5 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using USP5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
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Background
Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"