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USP5 antibody

Cat.#: 116688

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Product Information

  • Product Name
    USP5 antibody
  • Documents
  • Description
    USP5 Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in mouse lung tissue, HeLa cells, human brain tissue, MDA-MB-453s cells, rat brain tissue, rat lung tissue. Observed molecular weight by Western-blot: ~95 kDa
  • Tested applications
    ELISA, WB, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    Deubiquitinating enzyme 5 antibody; Isopeptidase T antibody; ISOT antibody; Ubiquitin thiolesterase 5 antibody; USP5 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of USP5 recombinant protein (Accession Number: NM_001098536). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

  • Validations

    mouse lung tissue were subjected to SDS PAGE followed by western blot with Catalog No:116688(USP5 antibody) at dilution of 1:400

    mouse lung tissue were subjected to SDS PAGE followed by western blot with Catalog No:116688(USP5 antibody) at dilution of 1:400

    IP Result of anti-USP5 (IP:Catalog No:116688, 4ug; Detection:Catalog No:116688 1:1000) with mouse brain tissue lysate 4000ug.

    IP Result of anti-USP5 (IP:Catalog No:116688, 4ug; Detection:Catalog No:116688 1:1000) with mouse brain tissue lysate 4000ug.

  • Background
    USP5, also named as ISOT, belongs to the peptidase C19 family. Knock-down of USP5 causes the accumulation of TP53/p53 and an increase in TP53/p53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated TP53/p53 but not with MDM2 for proteasomal recognition. USP5 is a potential target for p53 activating therapeutic agents for the treatment of cancer(PMID: 19098288). It is a novel proteasome associated protein(PMID: 19182904). USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers.
  • References
    • García-Caballero A, Gadotti VM, Stemkowski P. The deubiquitinating enzyme USP5 modulates neuropathic and inflammatory pain by enhancing Cav3.2 channel activity. Neuron. 83(5):1144-58. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"