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Product Name
USP33 antibody
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Description
USP33 Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in HEK-293 cells, mouse brain tissue. Positive IHC detected in human prostate cancer tissue, human kidney tissue. Observed molecular weight by Western-blot: 103 kDa,107 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Deubiquitinating enzyme 33 antibody; hVDU1 antibody; KIAA1097 antibody; Ubiquitin thiolesterase 33 antibody; USP33 antibody; VDU1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of USP33 recombinant protein (Accession Number: NM_201626). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:116607(USP33 antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human prostate cancer slide using Catalog No:116607(USP33 Antibody) at dilution of 1:50(under 10x lens)
Immunohistochemistry of paraffin-embedded human prostate cancer slide using Catalog No:116607(USP33 Antibody) at dilution of 1:50(under 40x lens)
IP Result of anti-USP33 (IP:Catalog No:116607, 4ug; Detection:Catalog No:116607 1:300) with HEK-293 cells lysate 2400ug.
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Background
USP33(Ubiquitin carboxyl-terminal hydrolase 33) is also named as KIAA1097, VDU1 and belongs to the peptidase C19 family. This protein, originally identified as a von Hippel-Lindau tumor suppressor (VHL) protein-interacting deubiquitinating enzyme, binds to the Robo1 receptor, and that USP33 is required for Slit responsiveness in breast cancer cells(PMID:19706539). The localization of USP33 is broadly confined to the secretory pathway, with all variants localizing to endoplasmic reticulum-associated structures(PMID:21801292). It has 3 isoforms produced by alternative splicing. This antibody is specific to USP33.
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References
- Wen P, Kong R, Liu J. USP33, a new player in lung cancer, mediates Slit-Robo signaling. Protein & cell. 5(9):704-13. 2014.
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