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Product Name
USP28 antibody
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Description
USP28 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells. Positive IP detected in HeLa cells. Positive IHC detected in human breast cancer tissue. Observed molecular weight by Western-blot: 135 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Deubiquitinating enzyme 28 antibody; KIAA1515 antibody; Ubiquitin thiolesterase 28 antibody; USP28 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of USP28 recombinant protein (Accession Number: NM_001346273). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116603(USP28 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:116603(USP28 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:116603(USP28 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-USP28 (IP:Catalog No:116603, 4ug; Detection:Catalog No:116603 1:600) with HeLa cells lysate 2800ug.
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Background
USP28(Ubiquitin carboxyl-terminal hydrolase 28) is also named as KIAA1515. USP28 is a 53BP1-interacting protein and a putative deubiquitinating enzyme, and belongs to a family of enzymes, called deubiquitinating enzymes (DUBs), which remove ubiquitin from the ubiquitin conjugates(PMID:16901786). It can be phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR(PMID:16901786). This protein has 2 isoforms produced by alternative splicing with the molecular mass of 122 kDa and 119 kDa.
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References
- Diefenbacher ME, Popov N, Blake SM. The deubiquitinase USP28 controls intestinal homeostasis and promotes colorectal cancer. The Journal of clinical investigation. 124(8):3407-18. 2014.
- Wu Y, Wang Y, Yang XH. The deubiquitinase USP28 stabilizes LSD1 and confers stem-cell-like traits to breast cancer cells. Cell reports. 5(1):224-36. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"