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Product Name
USP15 antibody
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Description
USP15 Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in mouse brain tissue, HEK-293 cells, HeLa cells. Positive IHC detected in human breast cancer tissue, human ovary tumor tissue. Observed molecular weight by Western-blot: 112 kDa
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Tested applications
ELISA, WB, IP, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Deubiquitinating enzyme 15 antibody; KIAA0529 antibody; Ubiquitin thiolesterase 15 antibody; Unph 2 antibody; UNPH4 antibody; USP15 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of USP15 recombinant protein (Accession Number: NM_001252079). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:50-1:500
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:116592(USP15 Antibody) at dilution of 1:1000
IP Result of anti-USP15 (IP:Catalog No:116592, 4ug; Detection:Catalog No:116592 1:1000) with mouse brain tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:116592(USP15 Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:116592(USP15 Antibody) at dilution of 1:200 (under 40x lens).
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Background
Ubiquitin is a highly conserved eukaryotic protein that is synthesized as a fusion protein precursor, either to itself or to one of two ribosomal proteins. USP15, is also named as KIAA0529,Unph-2, Unph4, encoding a human ubiquitin-specific protease (USP). The USP15 protein contains the highly conserved Cys and His boxes present in all members of he UBP family of deubiquitinating enzymes(PMID:10444327).It has 3 isoforms produced by alternative splicing.
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References
- Zou Q, Jin J, Hu H. USP15 stabilizes MDM2 to mediate cancer-cell survival and inhibit antitumor T cell responses. Nature immunology. 15(6):562-70. 2014.
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