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Product Name
USP14 antibody
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Description
USP14 Rabbit Polyclonal antibody. Positive WB detected in mouse kidney tissue, HeLa cells, mouse heart tissue, mouse liver tissue, mouse testis tissue. Positive IP detected in mouse liver tissue. Positive IF detected in HepG2 cells. Positive FC detected in HepG2 cells. Observed molecular weight by Western-blot: 52 kDa,60 kDa
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Tested applications
ELISA, WB, IF, FC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Deubiquitinating enzyme 14 antibody; TGT antibody; Ubiquitin thiolesterase 14 antibody; USP14 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of USP14 recombinant protein (Accession Number: NM_005151). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IF: 1:10-1:100
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Validations
mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:116591(USP14 antibody) at dilution of 1:300
Immunofluorescent analysis of HepG2 cells, using USP14 antibody Catalog No:116591 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-USP14 (IP:Catalog No:116591, 4ug; Detection:Catalog No:116591 1:1000) with mouse liver tissue lysate 4000ug.
1X10^6 HepG2 cells were stained with 0.2ug USP14 antibody (Catalog No:116591, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
USP14(Ubiquitin carboxyl-terminal hydrolase 14) is also named as TGT and belongs to the peptidase C19 family. Mammalian USP14 is unique among known UBP enzymes in that it is activated catalytically upon specific association with the 26S proteasome. USP14 inhibition accelerated the degradation of oxidized proteins and enhanced resistance to oxidative stress.This protein has a 45-kDa catalytic domain (PMID:16211010).
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