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Product Name
USP13 antibody
- Documents
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Description
USP13 Mouse Monoclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human testis tissue, human skin cancer tissue. Positive WB detected in HepG2 cells. Observed molecular weight by Western-blot: 97 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
Deubiquitinating enzyme 13 antibody; Isopeptidase T 3 antibody; IsoT 3 antibody; ISOT3 antibody; Ubiquitin thiolesterase 13 antibody; USP13 antibody
- Immunogen
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of USP13 recombinant protein (Accession Number: NM_003940). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:107533(USP13 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:107533(USP13 Antibody) at dilution of 1:200 (under 10x lens)
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:107533(USP13 Antibody) at dilution of 1:200 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:107533(USP13 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
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Background
USP13(Ubiquitin carboxyl-terminal hydrolase 13) is also named as ISOT3 and belongs to the peptidase C19 family. It is involved in ubiquitin-dependent protein catabolism. USP13 is responsible for MITF deubiquitination, utilizing a short hairpin RNA library against known deubiquitinating enzymes. This protein has 2 isoforms produced by alternative splicing.
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