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Product Name
USP12 Polyclonal Antibody
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Description
Polyclonal antibody to USP12
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
USP12 antibody; UBH1 antibody; USP12L1 antibody; ubiquitin specific peptidase 12 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic Peptide of human USP12.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
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Validations
Western blot - USP12 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using USP12 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 2min.
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Background
Deubiquitinating enzyme. Has almost no deubiquitinating activity by itself and requires the interaction with WDR20 and WDR48 to have a high activity. Not involved in deubiquitination of monoubiquitinated FANCD2. In complex with WDR48, acts as a potential tumor suppressor by positively regulating PHLPP1 stability.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"