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Product Name
USP11 antibody
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Description
USP11 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive IP detected in HEK-293 cells. Positive WB detected in human testis tissue, HEK-293 cells. Observed molecular weight by Western-blot: 100-110 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
Deubiquitinating enzyme 11 antibody; Ubiquitin thiolesterase 11 antibody; UHX1 antibody; USP11 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of USP11 recombinant protein (Accession Number: NM_001371072). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
human testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:116588(USP11 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:116588(USP11 antibody) at dilution of 1:100 (under 25x lens)
IP Result of anti-USP11 (IP:Catalog No:116588, 4ug; Detection:Catalog No:116588 1:300) with HEK-293 cells lysate 1480ug.
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Background
USP11(Ubiquitin carboxyl-terminal hydrolase 11), also named as UHX1, is a deubiquitinating enzyme controling many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. There are four bands of 120 kDa, 55 kDa, 50 kDa and 44 kDa to be detected in HeLa extract by western blot and the smaller proteins may be degradation products, some other spliced products could possibly correspond to the smaller proteins and possess functions(PMID:12084015). This protein belongs to the peptidase C19 family.
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