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Product Name
UQCRC2 Polyclonal Antibody
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Description
Polyclonal antibody to UQCRC2
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Tested applications
WB, IHC, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
UQCRC2 antibody; MC3DN5 antibody; QCR2 antibody; UQCR2 antibody; cytochrome b-c1 complex subunit 2, mitochondrial antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 224-453 of human UQCRC2 (NP_003357.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IP 1:50 - 1:200 -
Validations
Western blot - UQCRC2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using UQCRC2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
Western blot - UQCRC2 Polyclonal Antibody
Western blot analysis of extracts of various cells, using UQCRC2 antibody at 1:1000 dilution ratio through one-step method.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:4000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
Immunohistochemistry - UQCRC2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver injury using UQCRC2 antibody at dilution of 1:100 (40x lens).
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Background
This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. The core protein 2 is required for the assembly of the complex.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"