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Product Name
UQCRB antibody
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Description
UQCRB Rabbit Polyclonal antibody. Positive WB detected in human heart tissue, HeLa cells. Positive IP detected in mouse heart tissue. Positive IHC detected in human breast cancer tissue, human kidney tissue. Observed molecular weight by Western-blot: 14 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
Complex III subunit 7 antibody; Complex III subunit VII antibody; FLJ92016 antibody; FLJ97033 antibody; QCR7 antibody; QP C antibody; QPC antibody; UQBC antibody; UQBP antibody; UQCRB antibody; UQPC antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of UQCRB recombinant protein (Accession Number: NM_006294). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:116634(UQCRB antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:116634(UQCRB antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-UQCRB (IP:Catalog No:116634, 3ug; Detection:Catalog No:116634 1:600) with mouse heart tissue lysate 3200ug.
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Background
UQCRB(ubiquinol-cytochrome c reductase binding protein) is also named as UQBP, complex III subunit VII and belongs to the UQCRB/QCR7 family. It is a 13.4 kDa subunit of mitochondrial complex III, plays a crucial role in hypoxia-induced angiogenesis via mitochondrial reactive oxygen species (ROS)-mediated signaling(PMID:21215626). This protein has 2 isoforms produced by alternative splicing.
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References
- Li N, Bates DJ, An J, Terry DA, Wang E. Up-regulation of key microRNAs, and inverse down-regulation of their predicted oxidative phosphorylation target genes, during aging in mouse brain. Neurobiology of aging. 32(5):944-55. 2011.
- Tormos KV, Anso E, Hamanaka RB. Mitochondrial complex III ROS regulate adipocyte differentiation. Cell metabolism. 14(4):537-44. 2011.
- Villeneuve L, Tiede LM, Morsey B, Fox HS. Quantitative proteomics reveals oxygen-dependent changes in neuronal mitochondria affecting function and sensitivity to rotenone. Journal of proteome research. 12(10):4599-606. 2013.
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