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uPA antibody

Cat.#: 116622

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Product Information

  • Product Name
    uPA antibody
  • Documents
  • Description
    uPA Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human skin tissue, human lung tissue, human ovary tissue. Positive WB detected in HeLa cells, A375 cells, human brain tissue. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 54 kDa
  • Tested applications
    ELISA, WB, IHC, IP, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    ATF antibody; PLAU antibody; U plasminogen activator antibody; uPA antibody; URK antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of uPA recombinant protein (Accession Number: XM_047425353). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116622(uPA antibody) at dilution of 1:500

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116622(uPA antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human skin using Catalog No:116622(uPA antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human skin using Catalog No:116622(uPA antibody) at dilution of 1:50 (under 40x lens)

    IP Result of anti-uPA (IP:Catalog No:116622, 4ug; Detection:Catalog No:116622 1:1000) with HeLa cells lysate 2800ug.

    IP Result of anti-uPA (IP:Catalog No:116622, 4ug; Detection:Catalog No:116622 1:1000) with HeLa cells lysate 2800ug.

    Immunofluorescent analysis of HeLa cells using Catalog No:116622(uPA Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of HeLa cells using Catalog No:116622(uPA Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    PLAU(Urokinase-type plasminogen activator) is also named as uPA and belongs to the peptidase S1 family.UPA initiates a proteolytic cascade, which degrades extracellular matrix during tissue growth and remodelling.The secreted proform of UPA is activated after binding to a specific high affinity cell surface receptor. Secreted UPA is exposed to rapid inactivation in the pericellular space by plasminogen activator inhibitor-1 (PAI-1), which is stored in the extracellular matrix bound to vitronectin(PMID:9665342).It has 2 isoforms produced by alternative splicing.Defects in PLAU are the cause of Quebec platelet disorder (QPD).
  • References
    • Munakata S, Tashiro Y, Nishida C. Inhibition of plasmin protects against colitis in mice by suppressing matrix metalloproteinase 9-mediated cytokine release from myeloid cells. Gastroenterology. 148(3):565-578.e4. 2015.
    • Su B, Su J, He H. Identification of potential targets for diallyl disulfide in human gastric cancer MGC-803 cells using proteomics approaches. Oncology reports. 33(5):2484-94. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"