Rhesus IGFBP-2 / IGFBP2 (His Tag) recombinant protein

Rhesus IGFBP-2 / IGFBP2 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

IGFBP-2, also known as IGFBP2, is a insulin-like growth factor-binding protein (IGFBP). IGFBPs prolong the half-life of the IGFs , control bioavailability, activity, and distribution of insulin-like growth factor (IGF) through high-affinity IGFBP/IGF complexes. Six high-affinity IGF-binding proteins (IGFBP-1 to -6) have been identified. The six IGFBPs are structurally related but encoded by distinct genes. IGFBPs have a high affinity for IGFs. Some members of the IGFBP family have been consistently shown to inhibit IGF actions by preventing them from gaining access to the IGF receptors, while others potentiate IGF actions by facilitating the ligand-receptor interaction. IGFBP-2 is overexpressed in many malignancies and is often correlated with an increasingly malignant status of the tumor, pointing to a potential involvement of IGFBP-2 in tumorigenesis. It contains 1 IGFBP N-terminal domain and 1 thyroglobulin type-1 domain. It inhibits IGF-mediated growth and developmental rates.

Uncharacterized protein

IGF-BP53; MIGFBP-2; IGF-BINDING PROTEIN 2; IBP2; INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN 2; AI255832; IGFBP-2; IBP-2

F6YKM5

IGFBP2

Human Cells

A DNA sequence encoding the rhesus IGFBP2 (XP_001087071.1) (Met1-Gln324) was expressed, fused with a polyhistidine tag at the C-terminus.

Rhesus

The recombinant rhesus IGFBP2 comprises 300 amino acids and has a calculated molecular mass of 32.8 KDa.

> 67 % as determined by SDS-PAGE

Measured by its ability to inhibit the biological activity of IGFI or IGFII on MCF7 human breast adenocarcinoma cells (Karey, K.P. et al. (1988) Cancer Research 48:4083.).
The ED₅₀ for this effect is typically 0.02-0.1 μg/mL in the presence of 14 ng/mL human IGFII.