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Product Name
UGT1A9 Polyclonal Antibody
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Description
Polyclonal antibody to UGT1A9
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
UGT1A9 antibody; HLUGP4 antibody; LUGP4 antibody; UDPGT antibody; UDPGT 1-9 antibody; UGT-1I antibody; UGT1-09 antibody; UGT1-9 antibody; UGT1.9 antibody; UGT1A9S antibody; UGT1AI antibody; UGT1I antibody; UDPGT1-9 antibody; UDP-glucuronosyltransferase 1-9 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 26-250 of human UGT1A9 (NP_066307.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - UGT1A9 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using UGT1A9 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry - UGT1A9 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver cancer using UGT1A9 antibody at dilution of 1:200 (40x lens).
Immunofluorescence - UGT1A9 Polyclonal Antibody
Immunofluorescence analysis of MCF-7 cells using UGT1A9 antibody . Blue: DAPI for nuclear staining.
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Background
UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isoform has specificity for phenols. Isoform 2 lacks transferase activity but acts as a negative regulator of isoform 1.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"