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Product Name
UGT1A6 Polyclonal Antibody
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Description
Polyclonal antibody to UGT1A6
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
UGT1A6 antibody; GNT1 antibody; HLUGP antibody; HLUGP1 antibody; UDPGT antibody; UDPGT 1-6 antibody; UGT1 antibody; UGT1A6S antibody; UGT1F antibody; UDPGT1-6 antibody; UDP-glucuronosyltransferase 1-6 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 65-270 of human UGT1A6 (NP_001063.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IHC 1:50 - 1:100 -
Validations
Western blot - UGT1A6 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using UGT1A6 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
Western blot - UGT1A6 Polyclonal Antibody
Western blot analysis of extracts of HepG2 cells, using UGT1A6 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunohistochemistry - UGT1A6 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human uterine cancer using UGT1A6 antibody at dilution of 1:100 (40x lens).
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Background
UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isoform has specificity for phenols. Isoform 3 lacks transferase activity but acts as a negative regulator of isoform 1 (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"