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Product Name
UGT1A4 Polyclonal Antibody
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Description
Polyclonal antibody to UGT1A4
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
UGT1A4 antibody; HUG-BR2 antibody; UDPGT antibody; UDPGT 1-4 antibody; UGT-1D antibody; UGT1-04 antibody; UGT1.4 antibody; UGT1A4S antibody; UGT1D antibody; UDPGT1-4 antibody; UDP-glucuronosyltransferase 1-4 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UGT1A4 (NP_009051.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - UGT1A4 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using UGT1A4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
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Background
UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isoform glucuronidates bilirubin IX-alpha to form both the IX-alpha-C8 and IX-alpha-C12 monoconjugates and diconjugate. Isoform 2 lacks transferase activity but acts as a negative regulator of isoform 1 (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"