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Product Name
UFSP2 antibody
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Description
UFSP2 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human liver tissue, human brain tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue. Positive IP detected in HepG2 cells. Positive WB detected in mouse pancreas tissue, HeLa cells, HepG2 cells, NIH/3T3 cells. Observed molecular weight by Western-blot: 53kd
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
C4orf20 antibody; FLJ11200 antibody; UFM1 peptidase 2 antibody; Ufm1 specific protease 2 antibody; UFSP2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of UFSP2 recombinant protein (Accession Number: NM_018359). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with Catalog No:116552(UFSP2 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human liver using Catalog No:116552(UFSP2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:116552(UFSP2 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-UFSP2 (IP:Catalog No:116552, 3ug; Detection:Catalog No:116552 1:1000) with HepG2 cells lysate 1700ug.
Immunofluorescent analysis of HeLa cells using Catalog No:116552(UFSP2 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Ubiquitin-fold modifier 1 (Ufm1)-specific protease 2 (UfSP2) is a cysteine protease that is responsible for the release of Ufm1 from Ufm1-conjugated cellular proteins, as well as for the generation of mature Ufm1 from its precursor (PMID: 21228277). The MW of this protein is 53 kDa, and this antibody specially recognises the 53 kDa protein.
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