-
Product Name
UCP2 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to UCP2
-
Tested applications
WB, IHC
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
UCP2 antibody; BMIQ4 antibody; SLC25A8 antibody; UCPH antibody; uncoupling protein 2 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: A synthetic peptide of human UCP2
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IHC 1:20 - 1:200 -
Validations
Western blot - UCP2 Polyclonal Antibody
Western blot analysis of extracts of mouse spleen, using UCP2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
-
Background
UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"