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Product Name
UCK1 antibody
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Description
UCK1 Rabbit Polyclonal antibody. Positive WB detected in MCF7 cells. Positive IP detected in MCF-7 cells. Positive IF detected in MCF-7 cells. Observed molecular weight by Western-blot: 33 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Cytidine monophosphokinase 1 antibody; FLJ12255 antibody; UCK 1 antibody; UCK1 antibody; uridine cytidine kinase 1 antibody; Uridine monophosphokinase 1 antibody; URK1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of UCK1 recombinant protein (Accession Number: NM_001135954). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IF: 1:10-1:100
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:116675(UCK1 antibody) at dilution of 1:1000
IP Result of anti-UCK1 (IP:Catalog No:116675, 4ug; Detection:Catalog No:116675 1:1000) with MCF-7 cells lysate 2800ug.
Immunofluorescent analysis of MCF-7 cells using Catalog No:116675(UCK1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Uridine/cytidine kinase-1 is a pyrimidine ribonucleoside kinase that catalyzes the phosphorylation of uridine and cytidine to form uridine monophosphate (UMP) and cytidine monophosphate (CMP). The deduced 277-amino acid protein has a calculated molecular mass of 31 kDa. UCK1 belongs to the uridine kinase family and UCK have important roles for the phosphorylation of nucleoside analogs that may be important in chemotherapy of cancer.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"