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Product Name
UCHL5 antibody
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Description
UCHL5 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue, mouse brain tissue. Positive WB detected in mouse colon tissue, mouse brain tissue. Observed molecular weight by Western-blot: 38 kDa
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Tested applications
ELISA, IHC, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CGI 70 antibody; INO80R antibody; Ubiquitin thioesterase L5 antibody; UCH L5 antibody; UCH37 antibody; UCHL5 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of UCHL5 recombinant protein (Accession Number: NM_001199262). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
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Validations
mouse colon tissue were subjected to SDS PAGE followed by western blot with Catalog No:116673(UCHL5 Antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:116673(UCHL5 antibody) at dilution of 1:100 (under 10x lens)
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Background
UCHL5(Ubiquitin carboxyl-terminal hydrolase isozyme L5) is also named as UCH37 and belongs to the peptidase C12 family. It isfunctionally linked with multiple protein complexes and signal transduction pathways. Uch37’s weight average molecular weight increases as a function of its concentration. By size-exclusion chromatography, the apparent molecular weight of Uch37 was 51, 56, 70, and 109 kDa at 0.15, 0.69, 2.4, and 9.5 mg/mL, respectively. Given Uch37’s monomeric molecular weight of 37 kDa and its apparent elongated shape, its tendency toward an apparent molecular weight between 40 and 50 kDa at decreasing concentrations is appropriate for Uch37 monomers(PMID:21953935).
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