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Product Name
Mouse UBE2B/RAD6B recombinant protein
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Description
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.
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Protein name
Ubiquitin-conjugating enzyme E2 B
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Protein short names
RP23-263C23.2 ; MHR6B; HR6B; RAD6B; UBC2; E2-14K; HHR6B; RAD6B; HR6B; 2610301N02RIK; E2-17KDA; HHR6B; E2-14K
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Uniprot ID
P63146
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Gene Name
UBE2B; RAD6B
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
A DNA sequence encoding the mouse UBE2B (NP_003328.1) (Met1-Ser152) was expressed.
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Protein Species
Mouse
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Molecular weight
The recombinant mouse UBE2B consists 152 amino acids and predicts a molecular mass of 17.3 kDa.
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Purity
> 95 % as determined by SDS-PAGE.
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Validations
UBE2B protein SDS-PAGE
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