UBE2B antibody

Cat.#: 116516

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Product Information

  • Product Name
    UBE2B antibody
  • Documents
  • Description
    UBE2B Rabbit Polyclonal antibody. Positive WB detected in L02 cells, Transfected HEK-293 cells. Positive IHC detected in human ovary tumor tissue. Observed molecular weight by Western-blot: 17 kDa
  • Tested applications
    ELISA, IHC, WB
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    E2 17kDa antibody; HHR6B antibody; HR6B antibody; RAD6 homolog B antibody; RAD6B antibody; UBC2 antibody; UBE2B antibody; Ubiquitin carrier protein B antibody; Ubiquitin protein ligase B antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of UBE2B recombinant protein (Accession Number: NM_003337). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:10-1:100

  • Validations

    L02 cells were subjected to SDS PAGE followed by western blot with Catalog No:116516(UBE2B Antibody) at dilution of 1:600

    L02 cells were subjected to SDS PAGE followed by western blot with Catalog No:116516(UBE2B Antibody) at dilution of 1:600

    Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:116516(UBE2B antibody) at dilution of 1:10 (under 10x lens)

    Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:116516(UBE2B antibody) at dilution of 1:10 (under 10x lens)

  • Background
    UBE2B(Ubiquitin-conjugating enzyme E2 B) is also named as RAD6B and belongs to the ubiquitin-conjugating enzyme family. Rad6B is an E2-conjugating enzyme, reported to mediate beta-catenin polyubiquitination and stabilization, thus activating the Wnt signaling pathway(PMID:21118991). The gene encodes a 152 amino acid protein. The Rad6B and Cdc34 proteins are significantly elevated in meiotic and postmeiotic haploid germ cells when chromatin modi?cations occur(PMID:10373550).
  • References
    • Mou L, Zhang Q, Wang Y. Identification of Ube2b as a novel target of androgen receptor in mouse sertoli cells. Biology of reproduction. 89(2):32. 2013.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"