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Product Name
UBE2B antibody
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Description
UBE2B Rabbit Polyclonal antibody. Positive WB detected in L02 cells, Transfected HEK-293 cells. Positive IHC detected in human ovary tumor tissue. Observed molecular weight by Western-blot: 17 kDa
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Tested applications
ELISA, IHC, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
E2 17kDa antibody; HHR6B antibody; HR6B antibody; RAD6 homolog B antibody; RAD6B antibody; UBC2 antibody; UBE2B antibody; Ubiquitin carrier protein B antibody; Ubiquitin protein ligase B antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of UBE2B recombinant protein (Accession Number: NM_003337). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:10-1:100
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Validations
L02 cells were subjected to SDS PAGE followed by western blot with Catalog No:116516(UBE2B Antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:116516(UBE2B antibody) at dilution of 1:10 (under 10x lens)
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Background
UBE2B(Ubiquitin-conjugating enzyme E2 B) is also named as RAD6B and belongs to the ubiquitin-conjugating enzyme family. Rad6B is an E2-conjugating enzyme, reported to mediate beta-catenin polyubiquitination and stabilization, thus activating the Wnt signaling pathway(PMID:21118991). The gene encodes a 152 amino acid protein. The Rad6B and Cdc34 proteins are significantly elevated in meiotic and postmeiotic haploid germ cells when chromatin modi?cations occur(PMID:10373550).
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References
- Mou L, Zhang Q, Wang Y. Identification of Ube2b as a novel target of androgen receptor in mouse sertoli cells. Biology of reproduction. 89(2):32. 2013.
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