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Product Name
UBE4B antibody
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Description
UBE4B Rabbit Polyclonal antibody. Positive IHC detected in human heart tissue. Positive IF detected in HeLa cells. Positive WB detected in HeLa cells, mouse testis tissue, SH-SY5Y cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 146 kDa
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Tested applications
ELISA, IF, IHC, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
HDNB1 antibody; KIAA0684 antibody; UBE4B antibody; UBOX3 antibody; UFD2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of UBE4B recombinant protein (Accession Number: NM_006048). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116544(UBE4B Antibody) at dilution of 1:3000
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:116544(UBE4B Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:116544(UBE4B Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HeLa cells using Catalog No:116544(UBE4B Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
IP Result of anti-UBE4B (IP:Catalog No:116544, 4ug; Detection:Catalog No:116544 1:2000) with HeLa cells lysate 1200ug.
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Background
UBE4B represents ubiquitination factor E4B (UFD2 homolog, yeast)
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References
- Zhang H, Wu P, Chen F. SILAC-based quantitative proteomic analysis of secretome between activated and reverted hepatic stellate cells. Proteomics. 14(17-18):1977-86. 2014.
- Zhang XF, Pan QZ, Pan K. Expression and prognostic role of ubiquitination factor E4B in primary hepatocellular carcinoma. Molecular carcinogenesis. 55(1):64-76. 2016.
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