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Product Name
UBE3C Polyclonal Antibody
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Description
Polyclonal antibody to UBE3C
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Tested applications
WB, IHC, IF
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Species reactivity
Human
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Alternative names
UBE3C antibody; HECTH2 antibody; ubiquitin-protein ligase E3C antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human UBE3C (NP_055486.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100 -
Validations
Western blot - UBE3C Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using UBE3C antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - UBE3C Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human breast cancer using UBE3C Antibody at dilution of 1:100 (40x lens).
Immunofluorescence - UBE3C Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using UBE3C antibody . Blue: DAPI for nuclear staining.
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Background
E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'- or 'Lys-48'-linked polyubiquitin chains. Has preference for 'Lys-48' linkages. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"