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Product Name
UBE2V2 Polyclonal Antibody
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Description
Polyclonal antibody to UBE2V2
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
UBE2V2 antibody; DDVIT1 antibody; DDVit-1 antibody; EDAF-1 antibody; EDPF-1 antibody; EDPF1 antibody; MMS2 antibody; UEV-2 antibody; UEV2 antibody; ubiquitin conjugating enzyme E2 V2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-145 of human UBE2V2 (NP_003341.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - UBE2V2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using UBE2V2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - UBE2V2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat spleen using UBE2V2 antibody at dilution of 1:100 (40x lens).
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Background
Has no ubiquitin ligase activity on its own. The UBE2V2/UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"