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Product Name
UBE2U Polyclonal Antibody
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Description
Polyclonal antibody to UBE2U
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse
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Alternative names
UBE2U antibody; ubiquitin-conjugating enzyme E2 U antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-226 of human UBE2U (NP_689702.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100 -
Validations
Western blot - UBE2U Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using UBE2U antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunohistochemistry - UBE2U Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver using UBE2U antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - UBE2U Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using UBE2U antibody at dilution of 1:100 (40x lens).
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Background
Catalyzes the covalent attachment of ubiquitin to other proteins.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"